Older methods of assaying for protein:protein interactions in situ relied upon chemical cross-linking and antibodies. The obvious problem with these methods is that they alter the physiological conditions within the cell. Plus, they offer limited information on spatial distribution of the proteins in question. These problems can be solved by instead employing Fluorescence Resonance Energy Transfer (FRET) based techniques. Read the rest of this entry »

Rochelle Easton Esposito, working with the Genetics Society of America, has put together a spectacular set of video interviews with the founders of modern genetics. The series, “Conversations In Genetics“, provides an oral history of the last century of biology, and also serves as a larger commentary on how ideas evolve. What sets these interviews apart is that they’re each conducted by a prominent colleague, someone with a great depth of knowledge of the discoveries of the person being interviewed. These DVDs make wonderful teaching tools and are fascinating viewing for anyone interested in the process of science. Read the rest of this entry »

One of the most annoying things about trying out a new method is tracking down all of the reagents necessary to do the experiment. Having to dig through catalogs or search through websites can consume major amounts of time. To make CSH Protocols that much easier to use, we’re working with supply companies to put together reagent lists to save you time in preparing for your experiments. Read the rest of this entry »

CSH Protocols’ April Issue features a set of protocols from Michael Weber and colleagues at the University of Virginia detailing methods for using genetically engineered kinases to screen for novel protein kinase substrates. Determining the direct substrate of a protein kinase is important for understanding its function in vivo. Read the rest of this entry »

Our April issue is now online, and one of our featured protocols this month is a classic that, in the age of GFP and live imaging, has held up remarkably well and is still used with surprising frequency. Read the rest of this entry »