Came across this wonderful photo today (see it after the jump below), from Lewis Held’s book on Imaginal Discs. It’s from January, 1919, taken at a party to celebrate the return of Alfred Sturtevant from service in World War I. In it, you can see nearly all of the great fly pioneers, including T.H. Morgan, Calvin Bridges, Hermann Muller, Sturtevant, and one odd fellow you may or may not recognize.
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In situ hybridization of mRNA has long been a standard laboratory practice. Over recent years, the technique has evolved from the laborious sectioning of tissues and treatment with radioactive probes to the easier colorimetric (and occasionally fluorescent) methods now in use. Recent issues of CSH Protocols featured articles detailing in situ hybridization in Xenopus, Drosophila (here as well), and cultured cells.

November’s issue of CSH Protocols provides a full set of instructions for in situ hybridization on mouse embryos, as well as a cutting-edge method for imaging real-time gene expression in living systems with single-transcript resolution.

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