The baculovirus expression vector system has been widely used to produce proteins originating from both prokaryotic and eukaryotic sources. It offers easy cloning techniques and abundant viral propagation, and since it is based on an insect cell environment, it provides eukaryotic posttranslational modification machinery. Surface modifications of the viral capsid enable specific targeting. Such modifications can be used to enhance viral binding and entry to a wide variety of both dividing and nondividing mammalian cells, as well as to produce antibodies against the displayed antigen. In addition, the technology should enable modifications of intracellular behavior, i.e., trafficking of recombinant “nanoparticles,” a highly relevant feature for studies of targeted gene or protein delivery. In the March issue of Cold Spring Harbor Protocols, Christian Oker-Blom and colleagues provide a suite of articles detailing the use of baculovirus-based display and gene delivery systems. Their protocol for Creation of Baculovirus Display Libraries is a featured article for March, and is freely available, along with nearly 90 other featured articles.